Pengaruh Kerja Shift terhadap Kadar Glutamic Acid Decarboxylase 65 (GAD65) dalam Serum Darah

Imam Cahyo Murwidi


This study was to know shift work influences to increases levels of Glutamic Acid Decarboxylase 65 (GAD65) in the blood serum which is a marker of pancreatic beta cell destruction.The study method was quasi-experimental with the non-randomize pretest-posttest control group design. Subjects in this study were nurses at the R.A. Kartini Jepara hospital, female, aged 25 to 50 years, BMI 18.5 to 24.9, a work period of more than three years, did not have a routine or schedule a job outside the main working and randomize blood glucose less than 200 mg/dl. The sample size was 20 shift worker nurses and 20 non-shift worker nurses. GAD65 examination with ELISA method. The data study was analyzed with Wilcoxon test, paired t-test and independent t-test.The study results showed significant difference (p Wilcoxon test = 0.04) between the mean levels of GAD65 shift workers group pretest (0.189 ± 0.180 ng/ml) with posttest (0.334 ± 0.334 ng/ml). Not significant (p Paired t-test = 0.081) difference between the mean levels of GAD65 non-shift worker group pretest (0.326 ± 0.284 ng/ml) with posttest (0.218 ± 0.164 ng/ml). Significant (p Independent t-test = 0.008) difference between the mean delta GAD65 shift workers group (0.307 ± 0.145 ng/ml) with the non-shift workers group (- 0.107 ± 0.261 ng/ml).It can be concluded that shift work can increase GAD65 levels in blood serum but still within the normal range.


ELISA; GAD65; Shift work

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Boutin JA, Audinot V, Ferry G, Delagrange P. 2005. Molecular tools to study melatonin pathways and actions. Trends in Pharmacological Scienc, 26 (8):412-419.

Claustrat B, Brun J, Chazot G, 2005. The basic physiology and pathophysiology of melatonin. Sleep medicine reviews, 9(1), 11-24.

Fennel D. 2013. Night–Shift Work May Impair Glucosetolerance. http://www.diabetesselfmana- (Diakses pada 7 Oktober 2015).

Guyton AC, Hall JE. 2010. Textbook of medical physiology (12th ed). Philadelpia. Elsevier Inc.: 944-956

Hardeland, R. 2005. Atioxidative protection by melatonin. Endocrine, 27(2), 119-130.

Health and Safety Authority. 2012. Guidance for Employers and Employees on Night and Shift Work. H&SA.: 25

Hinke SA. 2007. Finding GAD: Early Detection of β-cell Injury. Endocrinology, 148(10): 4568–4571

Kaneto, H., Katakami, N., Matsuhisa, M., & Matsuoka, T. A. 2010. Role of reactive oxygen species in the progression of type 2 diabetes and atherosclerosis. Mediators of inflammation.

Lee JS, Kim YR, Park JM, Ha S, Kim YE, Baek NI, Hong EK. 2014. Mulberry fruit extract protects pancreatic β-cells against hydrogen peroxide-induced apoptosis via antioxidative activity. Molecules, 19: 8904-8915

Martinek B, 2013. Three Simple Ways That I Optimize My Sleep. (Diakses pada 23 Maret 2015).

Mirick DK, Bhatti P, Chen C, Nordt F, Stanczky FZ, Davis S. 2013. Night Shift Work and Levels of 6-Sulfatoxymelatonin and Cortisol in Men. Cancer Epidemiol Biomarkers Prev, 22 (6): 1079-1087.

Mullan CJM, Curhan GC, Schernhammer ES, Forman JP, 2012. Association of nocturnal melatonin secretion with insulin resistance in nondiabetic young women. American Journal of Epidemiology: 1-8.

Nurse Together, 2013. 5 Nutrition Tips for Night Shift Nurses. (Diakses pada 11 Oktober 2015).

Pan A, Schernhammer EA, Sun Q, Hu FB. 2011. Rotating night shift work and risk of type 2 diabetes: two prospective cohort studies in women. Plos Medicine, 8 (12): 1-9

Rajaratman SMW, Howard ME, Gruenstein RR. 2013. Sleep loss and circadian disruption in shift work: health burden and management. MJA, 199 (8): 511-515

Reed D. 2014. Healthy eating for healthy nurses: nutrition basics to promote for nurses and patients. OJIN, Vol. 7. No. 3

Reiter RJ, Tan DX, Osuna C, Gitto E. 2000. Actions of melatonin in the reduction of oxidative stress: A review. J Biomedical Research, 7: 444–58

Sharifian A, Farahani S, Pasalar P, Gharavi M, Aminian O. 2005. Shift work as on oxidative stressor. Journal of Circadian Rhythms, 3 (15): 1-3

Schlosser M, Walchus U, Kloting I, Walther R. 2008. Determination of glutamic acid decarboxylase (GAD65) in pancreatic islets and its in vitro and in vivo degradation kinetics in serum using a highly sensitive enzyme immunoassay. Disease Markers, 24: 191-198

Sheerwood L. 2010. Human Physiology: from Cells to Systems, 7th ed. United State: Ceangage Learning: 685-687

Seifert K, Tornow K, Walschus U, Kenk H, Schlosser M. 2011. Examination of GAD65 in human serum as a possible marker of ongoing beta cell destruction in autoimmune diabetes (abstract). Coe-Truman Technologies Inc.

Sengupta A, Tosini G. 2011. The Pineal Gland: a Neuroendocrine Transducer of Light Information. Atlanta: Circadian Rhythms and Sleep Disorders Program Neuroscience Institute and Department of Pharmacology and Toxicology Morehouse School of Medicine.

Steinbacher P, Ecki P. 2015. Impact oxidative Stress on Exercising Skeltal Muscle. Biomolecules, 5: 358.

Waldrop MA, Suckow AT, Marcovina SM, Chessler SD, 2007. Release of glutamate decarboxylase-65 into the circulation by injured pancreatic islet β cells. Endocrinology, 148(10): 4572–4578.

Yoon J, Jun H, 2005. Autoimmune destruction of pancreatic β cell. American Journal of Therapeutic, 12: 580-591.

Yonei Y, Hattori A, Tsutsui K, Okawa M, Ishizuka B, 2010. Effects of melatonin: basics studies and clinical applications. Anti-aging Medicine, 7 (7): 85-91.

Zhang L, Wei W, Xu J, 2006. Inhibitory effect of melatonin on diquat-induced lipid peroxidation in vivo as assessed by the measurement of F2-isoprostanes. J Pineal Research, 40: 326-331.



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