Efek Anti Inflamasi Ekstrak Daun Binahong [Anredera cordifolia (Ten.) Steenis] Topikal terhadap Jumlah PMN Neutrofil pada Tikus Jantan Sprague Dawley

Gita Susanti

Abstract


The purpose of this study was to determine the efficacy of binahong leaf extract ointment topically in a new incision wound healing compared to povidone iodine ointment. An experimental study was in vivo and was carried out using pre- and post-test group design from March to April 2015. The subject of the study was 30 Sprague Dawley White Male Rats divided into 5 groups. Group 1 was given base ointment, group 2 was given povidone iodine ointment, group 3 was given binahong leaf extract ointment 10%, group 4 was given binahong leaf extract ointment 20%, group 5 was given binahong leaf extract ointment 40%. The ointment was given 4 hours after incisions. The study data were tested with Levene Test, paired sample t-test, independent t-test, one-way ANOVA, and Post Hoc test using SPSS program version 20. The average concentrate of neutrophils before and after treatment with paired sample t-test showed a significant difference (p<0.05). Effectivity test between groups with independent t-test showed a significant difference except on a positive control group and binahong leaf extract 40%, Post Hoc test showed that there was a significant difference between applying binahong leaf extract ointment and povidone iodine ointment. In conclusion, binahong leaf extract ointment 40% has an anti-inflammatory effect in decreasing 81.25% of PMN neutrophil on incision wound but it less than povidone iodine ointment in decreasing 84.64% of PMN neutrophil.


Keywords


Anti-inflammatory; Binahong (Anredera cordifolia); Neutrophil

References


Arundina, I. 2003. Efek Anti inflamasi Catechin terhadap PMN yang Memfagosit Actinobacillus actynomycetemcomitan Penyebab Periodontitis. Majalah Kedokteran Gigi Dental Journal (Agustus: Edisi Khusus Temu Ilmiah Nasional III). Surabaya: Fakultas Kedokteran Gigi Universitas Airlangga.

Broughton II, G., Janis, J. E., Attiger, C. E. 2006. Wound Healing: an Overview. Plastic Reconstruction Surgery 117 (Supplement): 1-32.

Erwidawan, M. R. 2013. Pengaruh Konsentrasi Ekstrak Daun Binahong (Anredera cordifolia (Ten.) Steenis) terhadap Jumlah Pembuluh Darah pada Penyembuhan Luka Gingiva Tikus Putih (Rattus norvegicus). Skripsi, Universitas Gajah Mada Yogyakarta.

Farris, E. J. and Griffith, J. Q. 1971. The Rat in Laboratory Investigation. Hafner Publishing Company. New York. p. 408-411.

Hartono, E. A. 2011. Efek Ekstrak Etanol Daun Binahong (Anredera cordifolia (Ten.) Steenis) dalam Mempercepat Durasi Penyembuhan Luka Sayat pada Mencit Swiss Webster Jantan. Bandung: Fakultas Kedokteran Universitas Kristen Maranatha.

Mathivanan, N., Surendiran, G., Srinivasan, K., Malarvizhi, K. 2006. Morinda Pubescens J. E. Smith (Morinda tinctoria Roxb.) Fruit Extract Accelerates Wound Healing in Rats. Journal of Medicinal Food, Vol: 4. p. 591-593.

Muhammad, N. K. 2005. Antiseptics, Iodine, Povidone Iodine and Traumatic Wound Cleansing. Artikel Publikasi.

Raharjo, R. A. dan Eka, Y. 2011. Perbedaan Tingkat Penyembuhan Luka Tikus Wistar antara Luka yang Dibalut dengan Kassa Tulle dan yang Dibalut dengan Polyurethane Foam. Skripsi, Fakultas Kedokteran Universitas Diponegoro.

Reddy, G. A. K., Priyanka, B., Saranya, Ch. S., Kumar, C. K. A. 2012. Wound Healing Potential of Indian Medicinal Plants. International Journal of Pharmacy Review & Research, Vol: 2. p. 75-78.

Robbins, S. L. dan Kumar, V. 2007. Buku Ajar Patologi I. Edisi 7. Jakarta: EGC.

Soni, H. and Singhai, A. K. 2012. A Recent Update of Botanicals for Wound Healing Activity. International Research Journal of Pharmacy, 3. p. 1-6.




DOI: http://dx.doi.org/10.26630/jk.v8i3.644

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