Characteristics of Chicken Liver and Anchovy (Stolephorus indicus) Composite Powder to Prevent Stunting

Authors

  • Nawasari Indah Putri Sejati Nutrition Department, Poltekkes Kemenkes Tanjung Karang, Bandar Lampung, Indonesia
  • Endang Sri Wahyuni Nutrition Department, Poltekkes Kemenkes Tanjung Karang, Bandar Lampung, Indonesia
  • Bertalina Nutrition Department, Poltekkes Kemenkes Tanjung Karang, Bandar Lampung, Indonesia
  • Dina Fithriyani Faculty of Industrial Technology, Institut Teknologi Sumatera, Bandar Lampung, Indonesia

DOI:

https://doi.org/10.26630/jk.v17i1.5538

Keywords:

Animal protein, Anchovy powder, Chicken liver powder

Abstract

Stunting remains a major public health problem in Indonesia, with prevalence still exceeding national targets. Inadequate intake of high-quality animal protein during the first 1,000 days of life is a key contributing factor. Chicken liver and anchovies are affordable local sources of animal protein; however, their fresh forms are highly perishable. Powdered chicken liver and anchovies offer several advantages, including extended shelf life, greater storage practicality, and easier distribution. This study aimed to evaluate the physical, chemical, and sensory characteristics of chicken liver and anchovy composite powder stored for 4–6 weeks. An experimental study using a completely randomized design was conducted with three composite powder formulations: F1 (75% chicken liver:25% anchovy), F2 (50:50), and F3 (25:75). Physical properties (Hunter L*, a*, b*), proximate composition, and sensory characteristics were analyzed before and after storage. Data were analyzed using two-way repeated-measures ANOVA, LMM, and non-parametric tests (α=0.05). Results showed that formulation significantly affected color, texture, and proximate composition. Higher anchovy proportions increased brightness and smoothness. Storage influenced color intensity and aroma in certain formulations but did not affect texture stability. All formulations exhibited low moisture content (<10%) and high protein content (>60%). Protein levels remained stable after storage, while moisture, lipid, and carbohydrate contents were affected. Formula F3 consistently demonstrated superior nutritional and physical stability. With its extended shelf life and favorable nutritional value, the composite powder made from chicken liver and anchovies is expected to serve as a major protein source for stunting intervention, particularly in remote and underserved areas.

References

ABNT NBR ISO 20613. (2019). Sensory analysis - General guidance for the application of sensory analysis in quality control. 2019, 1–3. www.iso.org

Adu-Afarwuah, S., Lartey, A., Okronipa, H., Ashorn, P., Peerson, J. M., Arimond, M., Ashorn, U., Zeilani, M., Vosti, S., & Dewey, K. G. (2016). Small-quantity, lipid-based nutrient supplements provided to women during pregnancy and 6 mo postpartum and to their infants from 6 mo of age increase the mean attained length of 18-mo-old children in semi-urban Ghana: A randomized controlled trial. American Journal of Clinical Nutrition, 104(3), 797–808. https://doi.org/10.3945/ajcn.116.134692

Alghifari, I., Chandra, K., Muhammad, P., Rasyad, I., Zahran, F., Hidayat, F. Y., & Mutaqin, B. K. (2025). Tingkat Kecukupan Konsumsi Protein Hewani pada Masyarakat Dusun Margajaya dalam Upaya Pencegahan Stunting Adequacy of Animal Protein Consumption among the Margajaya Hamlet Community in Stunting Prevention. 7(2), 131–138. https://doi.org/10.24198/mktt.v7i2.68041

Ali, R. F. M. (2023). Nutritional quality and sensory properties of complementary food from taro flour, steamed lupine protein isolate powder, extra -virgin olive oil, and butternut squash flour. Applied Food Research, 3(2), 100321. https://doi.org/10.1016/j.afres.2023.100321

Amalia, R., Ramadani, A. L., & Muniroh, L. (2022). Hubungan Antara Riwayat Pemberian MP-ASI dan Kecukupan Protein dengan Kejadian Stunting pada Balita di Wilayah Kerja Puskesmas Bantaran Kabupaten Probolinggo. Media Gizi Indonesia, 17(3), 310–319. https://doi.org/10.20473/mgi.v17i3.310-319

Badan Standarisasi Nasional. (2005). Makanan Pendamping Air Susu Ibu (MP-ASI) - Bagian 1 : Bubuk Instan. Jakarta: Badan Standarisasi Nasional.

Bala, F. E., McGrattan, K. E., Valentine, C. J., & Jadcherla, S. R. (2024). A Narrative Review of Strategies to Optimize Nutrition, Feeding, and Growth among Preterm-Born Infants: Implications for Practice. Advances in Nutrition, 15(11), 100305. https://doi.org/10.1016/j.advnut.2024.100305

Bassey, F. I., Chinnan, M. S., Ebenso, E. E., Edem, C. A., & Iwegbue, C. M. A. (2013). Colour change: An indicator of the extent of maillard browning reaction in the food system. Asian Journal of Chemistry, 25(16), 9325–9328. https://doi.org/10.14233/ajchem.2013.15504

Baye, T. M., & Mekonone, S. T. (2025). Particle concentration and size effect on mechanical properties of natural-hydroxyapatite reinforced high-density polyethylene composite. 33, 1–12. https://doi.org/10.1177/09673911241313175

Binti, N. T., Hasan, M. M., Hossain, I., & Shikha, F. H. (2025). Evaluating the combined impact of sealing and temperature on the shelf-life of fish powder. LWT Food Science and Technology, 226(March), 117897. https://doi.org/10.1016/j.lwt.2025.117897

Bola, M. F. A., Latief, S., Lantara, A. M. H. D., Safitri, A., & Darma, S. (2024). FAKUMI MEDICAL JOURNAL Analisis Pelayanan Intervensi Gizi Spesifik pada Stunting di Wilayah Kerja Puskesmas. 04(11), 759–772. https://doi.org/10.33096/fmj.v4i11.514

Gbogbo, F., Arthur-Yartel, A., Bondzie, J. A., Dorleku, W. P., Dadzie, S., Kwansa-Bentum, B., Ewool, J., Billah, M. K., & Lamptey, A. M. (2018). Risk of heavy metal ingestion from the consumption of two commercially valuable species of fish from the fresh and coastal waters of Ghana. PLoS ONE, 13(3), 1–17. https://doi.org/10.1371/journal.pone.0194682

Giannakourou, M. C., Semenoglou, I., Arvaniti, M., Dermesonlouoglou, E., & Taoukis, P. (2025). Effect of Temperature and Water Activity on the Quality Kinetics of Packaged Fish Powder During Storage. Food and Bioprocess Technology, 18(10), 8482–8497. https://doi.org/10.1007/s11947-025-03931-5

Granato, D., Santos, J., Escher, G., Ferreira, B., & Maggio, R. (2017). Use of principal component analysis (PCA) and hierarchical cluster analysis (HCA) for multivariate association between bioactive compounds and functional properties in foods: A critical perspective. Trends in Food Science & Technology, 72. https://doi.org/10.1016/j.tifs.2017.12.006

Harrison, L., Padhani, Z., Salam, R., Oh, C., Rahim, K., Maqsood, M., Ali, A., Charbonneau, K., Keats, E. C., Lassi, Z. S., Imdad, A., Owais, A., Das, J., & Bhutta, Z. A. (2023). Dietary Strategies for Complementary Feeding between 6 and 24 Months of Age: The Evidence. Nutrients, 15(13). https://doi.org/10.3390/nu15133041

Hendrayati, Theresia Dewi, K. B., Budyghifari, L., & Adam, A. (2020). Proximate characteristics and nutritional value of white anchovy flour. Medico-Legal Update, 20(3), 744–749. https://doi.org/10.37506/mlu.v20i3.1490

Hunter Lab. (2025). Measuring the Color of Protein Powder Using Spectrophotometry Enhances Appeal. Blogpost. https://www.hunterlab.com/blog/measuring-the-color-of-protein-powder-using-spectrophotometry-enhances-appeal/

Khathir, R., Jannati, R., & Agustina, R. (2021). Estimasi Umur simpan. Jurnal Rona Teknik Pertanian, 14(2), 1–9. https://doi.org/10.17969/rtp.v14i2.20518

Kusuma, T. S., Kurniawati, A. D., Rahmi, Y., Rusdan, I. H., & Widyanto, R. M. (2017). Pengawasan Mutu Pangan (1st ed.). UB Press.

Lassi, Z. S., Das, J. K., Zahid, G., Imdad, A., & Bhutta, Z. A. (2013). Impact of education and provision of complementary feeding on growth and morbidity in children less than 2 years of age in developing countries: A systematic review. BMC Public Health, 13(SUPPL.3), S13. https://doi.org/10.1186/1471-2458-13-S3-S13

Li, P., Song, Z., Huang, L., Sun, Y., Sun, Y., Wang, X., & Li, L. (2023). Effects of Dietary Protein and Lipid Levels in Practical Formulation on Growth, Feed Utilization, Body Composition, and Serum Biochemical Parameters of Growing Rockfish Sebastes schlegeli. Aquaculture Nutrition, 2023. https://doi.org/10.1155/2023/9970252

Ministry of Health Republic Indonesia. (2018). Tabel Komposisi Pangan Indonesia. Jakarta: Ministry of Health Republic Indonesia. https://repository.kemkes.go.id/book/668

Ministry of Health Republic Indonesia. (2025). SSGI 2024 Dalam Angka. Jakarta: Health Development Policy Agency. https://www.badankebijakan.kemkes.go.id/survei-status-gizi-indonesia-ssgi-2024/

Moriconi, L., Vittadini, E., Linnemann, A. R., Fogliano, V., & Ngadze, R. T. (2023). Designing sustainable weaning foods for developing countries: not only a matter of nutrients. Food and Function, 14(20), 9194–9203. https://doi.org/10.1039/d3fo02832a

Mutamimah, D., Mahesvarah, T., & Envendi, J. (2023). Different Raw Material Effects In Preference Level And Moisture Content Of Seasoning Powder. Jurnal Ilmiah Kelautan Dan Perikanan, 21(1). https://doi.org/10.15578/chanos.v11i1.12773

National Agency of Drug and Food Control. (2024). Pedoman Penetapan Masa Simpan. Jakarta: National Agency of Drug and Food Control.

Nurpratama, W. L., Asmi, N. F., & Prakoso, A. D. (2024). Pengaruh intervensi pangan lokal dan konseling gizi terhadap stunting pada balita. Jurnal SAGO Gizi Dan Kesehatan, 5(3B), 1086. https://doi.org/10.30867/gikes.v5i3b.2177

Pawar, K., & Thompkinson, D. (2016). Changes in the Shelf Life Parameters of Dietary Supplement During Storage. Current Research in Nutrition and Food Science, 4(2). https://doi.org/10.12944/CRNFSJ.5.1.03

Pinto, C. F. D., Bortolo, M., Marx, F. R., & Trevizan, L. (2021). Characterisation of spray-dried hydrolysed chicken liver powder: effects on palatability and digestibility when included as a single source of animal protein in dog diets. Italian Journal of Animal Science, 20(1), 2086–2094. https://doi.org/10.1080/1828051X.2021.1993091

Putri CP, S Purwandini, & AC Adi. (2024). Pengaruh penambahan hati ayam, teri, dan kelor terhadap daya terima, gizi, serta nilai ekonomi kue beras. Jurnal Kesehatan Tambusai, 5, 2374–2384. https://journal.universitaspahlawan.ac.id/index.php/jkt/article/view/26044

Qu, Z., Tang, J., Sablani, S. S., Ross, C. F., Sankaran, S., & Shah, D. H. (2021). Quality changes in chicken livers during cooking. Poultry Science, 100(9), 101316. https://doi.org/10.1016/j.psj.2021.101316

Sejati, N. I. P., Mulyani, R., Lupiana, M., & Wahyuni, E. S. (2025). Karakteristik Kimia Tepung Hati Ayam Ikan Teri Sebagai Bahan Pembuat MPASI dalam Pencegahan dan Penanggulangan Stunting. Jurnal Ilmiah Obsgin, 17(1), 8–15. https://stikes-nhm.e-journal.id/JOB/article/view/2568/2200

Sitepu, M., Rahmawati, W., & Kuncoro, S. (2022). Jurnal Agricultural Biosystem Engineering: Studying the Characteristics of Thin-Layer Drying of Chicken Innards. 1(3), 319–330. https://doi.org/10.23960/jabe.v1i3.6323

Utami, F., Wahyuni, E. S., Putri Sejati, N. I., & Ramadanti, R. O. (2025). Evaluasi proksimat formula Makanan Pendamping ASI berbasis tepung hati ayam dan ikan teri untuk pencegahan stunting pada balita. Jurnal SAGO Gizi Dan Kesehatan, 6(2), 411. https://doi.org/10.30867/gikes.v6i2.2638

Wahyuni, E. S., Sejati, N. I. P., Muliani, U., & Bertalina. (2024). Organoleptik Mpasi Tepung Hati Ayam Ikan Teri Dalam Pencegahan Stunting. PREPOTIF : Jurnal Kesehatan Masyarakat, 8(1), 393–345. https://journal.universitaspahlawan.ac.id/index.php/prepotif/article/view/25773

Wardani, W. V., Hadi, R., Fauziyah, N. R., & Zakaria, M. (2025). Potensi dan risiko taburia hati ayam sebagai upaya pencegahan stunting dan defisiensi vitamin A pada balita. Jurnal SAGO Gizi Dan Kesehatan, 6(1), 35. https://doi.org/10.30867/gikes.v6i1.1875

World Health Organization. (2023). WHO Guideline for complementary feeding of infants and young children 6-23 months of age. Geneva: WHO.

Windya Kusumaningtyas, R., Laily, N., & Sudiarti, T. (2024). Nutritional and functional characterization of the anchovy (Stolephorus indicus) powder produced by conventional drying and foam mat drying. BIO Web of Conferences (Vol. 98, p. 01001). EDP Sciences. https://doi.org/10.1051/bioconf/20249801001

Yadav, U., Singh, R. R. B., & Arora, S. (2018). Evaluation of quality changes in nutritionally enriched extruded snacks during storage. Journal of Food Science and Technology, 55(10), 3939–3948. https://doi.org/10.1007/s13197-018-3319-3

Yian, L. Y., & Phing, P. L. (2020). Storage stability of kuini powder in two packaging types: aluminum laminated polyethylene and polyethylene terephthalate. Malaysian Journal of Analytical Sciences, 24(5), 657–669. https://mjas.analis.com.my/mjas/v24_n5/pdf/Loo_24_5_4.pdf

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Published

03-06-2026

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Vol. 17 No. 1 (2026): Jurnal Kesehatan

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Copyright (c) 2026 Nawasari Indah Putri Sejati, Endang Sri Wahyuni, Bertalina, Dina Fithriyani

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