Aktivitas Lactobacillus plantarum Isolat Air Susu Ibu pada Tikus Galur Wistar Diabetes Mellitus

Oktavia Kartikasari, Anggun Dian Astuti, Mega Berkah Mustika Wabula, Sri Sinto Dewi

Abstract


Lactobacillus plantarum is a Lactic Acid Bacteria (LAB) that has the potential to be used as a probiotic agent. Probiotics are antidiabetic because they can inhibit alpha-glucosidase enzymes. This study aims to was to determine the antidiabetic effects of probiotic Lactobacillus plantarum isolates of breast milk (ASI). The study was conducted at the Microbiology Laboratory of the University of Muhammadiyah Semarang and the Pharmacology Laboratory of the Semarang College of Pharmacy during the month of April-June. Experimental research with 25 male wistar mice was divided into 5 groups (K-, K+, P1, P2, P3) Alloxan monohydrate induction was carried out intraperitoneally at a dose of 150mg/kg BW. Fasting blood glucose measurements were performed at baseline (before alloxan induction), post alloxan and at the end of treatment. Diabetic mice fasting blood glucose levels shouldbe± 200 mg/dL. Suspension of Lactobacillus plantarum isolate ASI was carried out orally for 7 days. Based on the ANOVA test there were significant differences in the decrease in blood glucose with a significant value of p-value=0,009<0,05. Based on the Tuckey post hoc test treatment group P1, P2, P3 there were no significant differences between treatment groups even though the dosage was different. 1 time a day treatment is an efficient dose in reducing blood glucose.


Keywords


Antidiabetic, Breast Milk; Diabetes Mellitus; LAB; Lactobacillus plantarum

Full Text:

PDF

References


American Diabetes Association (ADA). (2011). Diagnosis and Classification of Diabetes Mellitus. Diabetes Care. Jan;34 (Suppl 1): S62-S69.

Adewani, N. (2008). Pengaruh Pemberian Rebusan Kulit Kayu Duwet Terhadap Persentase Penurunan Kadar Glukosa Darah Pada Tikus Putih. [Skripsi]. Yogyakarta: Universitas Muhammadiyah Yogyakarta.

Alsayadi, M., et al. (2014). Evaluation of Anti-Hyperglycemic and Anti-Hyperlipidemic Activities of Water Kefir as Probiotic on Streptozotocin-Induced Diabetic Wistar Rats’, Journal of Diabetes Mellitus, 04(02), pp. 85-95. doi: 10.4236/jdm.2014.42015.

Amaretti A, di Nunzio M, Pompei A, Raimondi S, Rossi M, Bordoni A. (2013). Antioxidant properties of potentially probiotic bacteria: in vitro and in vivo activities. Appl Microbiol Biotechnol. 97(2):809-817.

Atlas, I. D. (2015). International Diabetes Federation. ISBN 2930229853, 7.

Chen, P. et al. (2014). Screening for potential new probiotic based on probiotic properties and α-glucosidase inhibitory activity. Food Control. Elsevier Ltd, 35(1), pp. 65-72. doi: 10.1016/j.foodcont.2013.06.027.

Dewi, S.S & Herlisa, A. (2015). Aktivitas Lactobacillus plantarum Isolat ASI Terhadap Imunoglobulin (IgA , IgG) pada Tikus Wistar Model Sepsis. The 2nd University Research Coloquium 2015. ISSN 2407-9189.

Dewi, S. S. and Ariyadi, T. (2017) Profil Protein Bakteri Asam Laktat Isolat Air Susu Ibu. Prosiding Seminar Nasional & Internasional. Semarang: Universitas Muhammadiyah Semarang.

Djide M.N, Wahyudin E. (2008). Isolasi Bakteri Asam Laktat dari Air Susu Ibu, dan Potensinya dalam penurunan kadar kolesterol secara in vitro. Majalah Farmasi dan Farmakologi, Vol. 12, No. 3- Nopember 2008 (ISSN:1410- 7031).

Fadhilah, A.N., Hafsan,. Famawati, N. (2015) Penurunan Kadar Kolesterol Oleh Bakteri Asam Laktat Asal Dangke Secara In Vitro. Prosiding Seminar Nasional Mikrobiologi Kesehatan Dan Lingkungan. Makassar. 29 Januari 2015. ISBN 978-602-722450-6.

Fatmawati. (2013). Potensi Isolat Bakteri Asam Laktat Yang Diisolasi dari Dangke Sebagai Probiotik Berdasarkan Toleransi pH Rendah. [Skripsi]. Makassar: Fakultas Sains dan Teknologi, UIN Alauddin Makassar.

Gao, H. et al. (2007). Inhibitory effect on α-glucosidase by the fruits of Terminalia chebula Retz, Food Chemistry, 105(2), pp. 628-634. doi: 10.1016/j.foodchem.2007.04.023.

Jain, S. and Saraf, S. (2010) Type 2 diabetes mellitus-Its global prevalence and therapeutic strategies. Diabetes and Metabolic Syndrome: Clinical Research and Reviews. Diabetes India, 4(1), pp. 48-56. doi: 10.1016/j.dsx.2008.04.011.

Januarsyah,T. (2007). Kajian Aktivitas Hambat Bakteriosin dari Bakteri Asam Laktat Galur SCG 1223. [Skripsi]. Bogor: Fakultas Teknologi Pertanian Institut Pertanian Bogor.

Lutgendorff F, Trulsson LM, van Minnen LP, Rijkers GT, Timmerman HM, Franzen LE, Gooszen HG, Akkermans LMA, Soderholm JD. (2008). Probiotics enhance pancreatic glutathione biosynthesis and reduce oxidative stress in experimental acute pancreatitis. Am J Physiol Gastrointest Liver Physiol. 295:G1111–G1121.

Lutgendorff F, Nijmeijer RM, Sandstrom PA, Trulsson LM, Magnusson KE, Timmerman HM, van Minnen LP, Rijkers GT, Gooszen HG, Akkermans LA, Soderholm JD. (2009). Probiotics prevent intestinal barrier dysfunction in acute pancreatitis in rats via induction of ileal mucosal glutathione biosynthesis. PLoS ONE. 4(2):e4512.

Lye, H. S. et al. (2009). The improvement of hypertension by probiotics: Effects on cholesterol, diabetes, renin, and phytoestrogens. International Journal of Molecular Sciences, 10(9), pp. 3755–3775. doi: 10.3390/ijms10093755.

Malik, I. M., Nasrul, E. and Asterina. (2014) ‘Hubungan Hiperglikemia dengan Prothrombin Time pada Mencit (Mus musculus) yang Diinduksi Aloksan’, Jurnal Kesehatan Andalas, 4(1), pp. 182–188.

Marianne et al. (2014). Antidiabetic Activity of Leaves Ethanol Extract Chromolaena odorata (L.) R.M. King on Induced Male Mice with Alloxan Monohydrate. Jurnal Natural, 14(1), pp. 1-4. doi: 10.24815/jn.v14i1.1382.

Panwar, H. et al. (2014). Lactobacillus strains isolated from infant faeces possess potent inhibitory activity against intestinal alpha- and beta-glucosidases suggesting anti-diabetic potential. European Journal of Nutrition, 53(7), pp. 1465-1474. doi: 10.1007/s00394-013-0649-9.

Purwanti, H. S. (2004). Konsep penerapan ASI eksklusif. Jakarta: EGC.

Rahayu, E. S. (2001). Potensi dan peranan prebiotik dan probiotik dalam makanan sehat. Seminar Prebiotik, Probiotik dan Makanan Sehat. Fakultas Biologi Universitas Atmajaya. Yogyakarta.

Ramchandran, L. and Shah, N. P. (2008). Proteolytic profiles and angiotensin-I converting enzyme and α-glucosidase inhibitory activities of selected lactic acid bacteria. Journal of Food Science, 73(2), pp. 75-81. doi: 10.1111/j.1750-3841.2007.00643.x.

Sari, P. P. et al. (2017). Lactobacillus casei Fermented Milk as a Treatment for Diabetes in Mice (Mus musculus). Jurnal Medika VeterinariaA & A (Banda Aceh), 11(1), pp. 15-19. doi: 10.21157/j.med.vet..v11i1.4088.

Salminen, S. & A. V. Wright. (2004). Lactic Acid Bacteria. Microbiology and Functional Aspects. 2nd Edition, Revised and Expanded. Marcell Dekker, Inc., New York.

Subroto, A. (2006). Ramuan Herbal untuk Diabetes Melitus. Depok: Penebar Swadaya.

Setianingsih, S. (2010). Kajian Senyawa Antimikroba Bakteri Asam Laktat Homofermentaatif Isolat ASI. Journal of Chemical Information and Modeling. doi: 10.1017/CBO9781107415324.004.

Syafiqoh, N. (2016). Aktivitas Antioksidan dan Efek Antidiabetes Probiotik Lactobacillus plantarum SK(5) Asal Bekasam. [Tesis]. Bogor: Program Studi Mikrobiologi, Sekolah Pascasarjana, Institut Pertanian Bogor.

Szkudelski, T. (2001). The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiological Research, 50(6), pp. 537–546.

Walde, S.S., Dohle, C., Schott-Ohly, P., Gleichmann, H. (2002). Molecular target structures in alloxan-induced diabetes in mice. Life Sciences, 71, 1681-1694.

World Health Organization. (2016). Diabetes Fakta dan Angka. 26 November 2018. http://www.searo.who.int/indonesia/topics/8-whd2016-diabetes-facts-andnumbers-indonesian.pdf.

Yun, S. I., Park, H. O. and Kang, J. H. (2009) Effect of Lactobacillus gasseri BNR17 on blood glucose levels and body weight in a mouse model of type 2 diabetes. Journal of Applied Microbiology, 107(5), pp. 1681-1686. doi: 10.1111/j.1365-2672.2009.04350.x.

Zhang Y, Zhang H. (2013). Microbiota associated with type 2 diabetes and its related complications. Food Sci Hum Well. 2:167-172.




DOI: https://doi.org/10.26630/jk.v10i3.1488

Refbacks

  • There are currently no refbacks.


Published by: Poltekkes Kemenkes Tanjung Karang

Alamat: Jl. Soekarno-Hatta No. 6 Bandar Lampung
Telepon: 0721-783852 Fax: 0721-773918, emai: jk@poltekkes-tjk.ac.id
 

Jurnal Kesehatan is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.