Effect of Green Betle Leaf Extract (Piper betle L.) on Indoor Microbiological Quality

Authors

  • Ratih Mutiara Nugraheni Politeknik Kesehatan Kementerian kesehatan Yogyakarta
  • Sigid Sudaryanto Politeknik Kesehatan Kementerian kesehatan Yogyakarta
  • Naris Dyah Prasetyawati Politeknik Kesehatan Kementerian kesehatan Yogyakarta
  • Tri Mulyaningsih Politeknik Kesehatan Kementerian kesehatan Yogyakarta

DOI:

https://doi.org/10.26630/rj.v18i3.4566

Keywords:

Bacteria, Disinfection, Humidity, Indoor, Piper betle L, Air

Abstract

The microbiological quality of indoor air is still a signifikans concern because it causes mortality and morbidity. This study aims to analyze the effect of green betel leaf extract (Piper betle L.) on reducing the bactherial in the air. Quasi-research using the One Group Pretest Posttest design, conducted in March-May 2024. Betle leaves were extracted using the maceration method using 96% Ethanol solvent, then diluted with distilled water to obtain a concentration of 15%. Three volume variations (0.5 ml, 1.0 ml, and 1.5 ml) were put into a humidifier and applied to a nine-square-meter bedroom for three hours, with six replication. Sampling was carried out before and after the experiment. All data (N= 48) were analyzed using the One Way ANOVA and Tukey tests at CL 95%. The study results found a decrease in colonies in the treatment with a volume of 1.0 ml and 1.5 ml: 39.3% (from 36.2 to 22.3 CFU /m3) and 17.6% (from 49.2 to 37.0 CFU /m3). The treatment with a volume of 0.5 ml did not show a decrease. The statistical analysis showed a significant effect of betle leaf extract on reducing the bacteria in the air (P= 0.020). The study results have proven that green betle leaf extract can be used as a disinfectant to reduce the bacteria in the air. However, safe use must be applied by paying attention to the air humidity level.

References

Akbar, F., Vera, O., Askur, A., & Mappau, Z. (2023). Kemampuan Ekstrak Simplisia Daun Pandan Wangi (Pandanus Sp) dalam Mengendalikan Lalat Rumah (Musca Domestica) dengan Penggunaan Air Humidifier Ultrasonic. Suara Forikes: Journal of Health Research, 14(4), 709–713. https://doi.org/10.33846/sf14410

Andila, P. S., Warseno, T., Li’aini, A. S., Tirta, I. G., Wibawa, I. P. A. H., & Bangun, I. P. A. H. W. M. (2020). Seri Koleksi Kebun Raya Eka Karya Bali Tanaman Berpotensi Penghasil Minyak Atsiri. LIPI Press, anggota Ikapi. https://doi.org/https://doi.org/10.14203/press.311

Andriana, Y., Widodo, A. D. W., & Endraswari, P. D. (2023). A Correlation between the Number of Airborne Bacteria and Fungi using the Settle Plate Method with Temperature and Relative Humidity at the Clinical Microbiology Laboratory of Dr. Soetomo Regional General Hospital Surabaya, Indonesia. Journal of Pure and Applied Microbiology, 17(2), 942–950. https://doi.org/10.22207/JPAM.17.2.24

Apte, K., & Salvi, S. (2016). Household air pollution and its effects on health. F1000Research, 5, 2593. https://doi.org/10.12688/f1000research.7552.1

Aurora, W. I. D. (2021). Efek Indoor Air Pollution Terhadap Kesehatan. Electronic Journal Scientific of Environmental Health And Disease, 2(1), 32–39. https://doi.org/10.22437/esehad.v2i1.13750

Bahri, B., Raharjo, M., & Suhartono, S. (2021). Dampak Polusi Udara Dalam Ruangan Pada Kejadian Kasus Pneumonia: Sebuah Review. Link, 17(2), 99–104. https://doi.org/10.31983/link.v17i2.6833

Broto, W., Arifan, F., Fatimah, S., & Alwy, L. N. (2021). Desinfektan dari Batang Serai , Daun Serai , Daun Sirih dan Kulit Jeruk Nipis. Pentana, 02(3), 12–16. https://ejournal2.undip.ac.id/index.php/pentana/article/view/14705

Chan, C., & Holosko, M. J. (2020). Utilizing Youth Media Practice to Influence Change: A Pretest–Posttest Study. Research on Social Work Practice, 30(1). https://doi.org/https://doi.org/10.1177/1049731519837357

Cui, J., Chen, C., Gan, Q., Wang, T., Li, W., Zeng, W., Xu, X., Chen, G., Wang, L., & A, Z. L. (2022). Indoor microplastics and bacteria in the atmospheric fallout in urban homes. Science of The Total Environment, 852(0048–9697). https://doi.org/https://doi.org/10.1016/j.scitotenv.2022.158233

Dewi, W. C., Raharjo, M., & Wahyuningsih, N. E. (2021). Literatur Review : Hubungan Antara Kualitas Udara Ruang Dengan Gangguan Kesehatan Pada Pekerja. An-Nadaa: Jurnal Kesehatan Masyarakat, 8(1), 88. https://doi.org/10.31602/ann.v8i1.4815

Eun-Jung Yoon, Jeong, J., Yoon, E., & Park, D. (2023). The effects of treadmill exercise on brain angiogenesis in ovariectomized rats. Physiological Reports, 11(21). https://doi.org/https://doi.org/10.14814/phy2.15864

Hany, A., Ariningpraja, R. T., Ismail, D. D. S. L., Kristianto, H., Kartika Sari, E., Fatma, E. P. L., Wibisono, A. H., Lukitasari, M., & Sari, D. P. (2021). Peningkatan Pengetahuan Perawat Tentang Penggunaan Terapi Oksigen Non Humidifier Pada Pasien Dengan Nasal Kanul Di Rsud Dr. Saiful Anwar. Caring Jurnal Pengabdian Masyarakat, 1(1), 21–29. https://doi.org/10.21776/ub.caringjpm.2021.001.01.3

Hermanto, L. O., Nibenia, J., Sharon, K., & Rosa, D. (2023). Pemanfaatan Tanaman Sirih (Piper betle L) Sebagai Obat Tradisional. Pharmaceutical Science Journal, 3(1), 33–42. https://doi.org/http://dx.doi.org/10.52031/phrase.v3i1.502

Kemenkes RI. (2023). Peraturan Menteri Kesehatan Republik Indonesia Nomor 2 Tahun 2023 Tentang Kesehatan Lingkungan.

Kopong, M. V. U., & Warditiani, N. K. (2022). Review artikel : Potensi daun sirih hijau (piper betle l.) dan daun sirih merah (piper crocatum) sebagai antioksidan. Jurnal Ilmiah Multi Disiplin Indonesia, 2(3), 710–729. https://doi.org/https://doi.org/10.32670/ht.v2iSpesial%20Issues%203.1504

Landrigan, P. J. (2017). Air pollution and health. The Lancet Public Health, 2(1), e4–e5. https://doi.org/10.1016/S2468-2667(16)30023-8

Lee, E., Lee, S.-Y., & Hong, S.-J. (2020). The past, present, and future of humidifier disinfectant-associated interstitial lung diseases in children. Clinical and Experimental Pediatrics, 63(7), 251–258. https://doi.org/10.3345/cep.2019.01326

Lee, K. K., Bing, R., Kiang, J., Bashir, S., Spath, N., Stelzle, D., Mortimer, K., Bularga, A., Doudesis, D., Joshi, S. S., Strachan, F., Gumy, S., Adair-Rohani, H., Attia, E. F., Chung, M. H., Miller, M. R., Newby, D. E., Mills, N. L., McAllister, D. A., & Shah, A. S. V. (2020). Adverse health effects associated with household air pollution: a systematic review, meta-analysis, and burden estimation study. The Lancet Global Health, 8(11), e1427–e1434. https://doi.org/10.1016/S2214-109X(20)30343-0

Lidya, E., & Yushananta, P. (2024). Effects of Frangipani Flower Extract (Plumeria acuminata L.) Against the Mortality of Aedes aegypti Larvae. Ruwa Jurai: Jurnal Kesehatan Lingkungan, 18(1), 56–65. https://doi.org/10.26630/rj.v18i1.4513

Marsden, E., & Torgerson, C. J. (2012). Single group, pre- and post-test research designs: Some methodological concerns. Oxford Review of Education, 38(5), 583–616. https://doi.org/10.1080/03054985.2012.731208

Muhammad, A., & Widayati, S. (2024). Komparasi Media Kultur Bakteri Pemeriksaan Angka Kuman Ruang Pada Metode Settle Plate. Berkala Ilmiah Kedokteran Dan Kesehatan Masyarakat (Scientific Periodical Journal Of Medicine And Public Health), 2(2). https://doi.org/10.20885/bikkm.vol2.iss2.art7

Mustam, M., Azis, H. A., & Alam, R. (2022). Aktivitas Antibakteri Disinfektan Ekstrak Daun Sirih dan Jeruk Nipis Terhadap Bakteri Staphylococcus. a dan E.coli. JURNAL TECNOSCIENZA, 6(2), 219–233. https://doi.org/10.51158/tecnoscienza.v6i2.624

Nie, X. L., Ouyang, C. Y., & Ning, L. (2014). Research on Material Structure, Appearance and Color Design and Application of Humidifiers - With Student Consumers as Research Objects. Advanced Materials Research, 936, 1882–1885. https://doi.org/10.4028/www.scientific.net/AMR.936.1882

Nugroho, F., Oktavianthi, A. T., & Bani, A. U. (2022). Rancang Bangun Robot Humidifier Beroda Untuk Menjaga Kelembapan Udara Ideal Mencegah Terinfeksi Bakteri Berbasis Mikrokontroler. Building of Informatics, Technology and Science (BITS), 4(2), 1091–1103. https://doi.org/10.47065/bits.v4i2.1977

Park, D.-U., Choi, Y.-Y., Ahn, J.-J., Lim, H.-K., Kim, S.-K., Roh, H.-S., Cheong, H.-K., Leem, J.-H., Koh, D.-H., Jung, H.-J., Lee, K.-M., Lee, J.-H., Kim, Y.-H., Lim, S.-Y., Paek, D.-M., Lim, C.-M., & Hong, S.-J. (2015). Relationship between Exposure to Household Humidifier Disinfectants and Risk of Lung Injury: A Family-Based Study. PLOS ONE, 10(5), e0124610. https://doi.org/10.1371/journal.pone.0124610

Romano, S. (2023). How Your Home’s Humidity Affects Indoor Air Quality. Iaq.Works. https://iaq.works/humidity/how-your-homes-humidity-affects-indoor-air-quality/

Rosyad, M. N., Makkadafi, S. P., & Kusumawati, N. (2024). Perhitungan Kuman Udara Pada Ruang ICU dan Ruang HD Di Rumah Sakit Kota Samarinda Metode Settle Down. Borneo Journal of Medical Laboratory Technology, 7(1), 591–597. https://doi.org/10.33084/bjmlt.v7i1.7613

Sadiah, H. H., Cahyadi, A. I., & Windria, S. (2022). Kajian Daun Sirih Hijau (Piper betle L) Sebagai Antibakteri. Jurnal Sain Veteriner, 40(2), 128. https://doi.org/10.22146/jsv.58745

Sari, M. L. N., Fikri, A., Murwanto, B., & Yushananta, P. (2022). Analisis Faktor Lingkungan Fisik Dan Kejadian Tuberkulosis di Wilayah Kerja Puskesmas Kedaton Kota Bandar Lampung. Ruwa Jurai: Jurnal Kesehatan Lingkungan, 16(3), 152. https://doi.org/10.26630/rj.v16i3.3629

Syaputri, D., Tanjung, R., Yuniastuti, T., Rahmawati, Syaiful, A. Z., Patilaiaya, H. La, Manalu, S. M. H., S, T. T. B., Buamona, S. A. M. ., & Suhartawan, B. (2022). Penyehatan Udara. PT. GLOBAL EKSEKUTIF TEKNOLOGI.

Trigunarso, S. I., Yushananta, P., & Ainin, F. K. (2018). Kadar Debu terhadap Kapasitas Vital Paru pada Masyarakat di Sekitar PT Semen Baturaja. Jurnal Kesehatan, 9(3), 396–402. https://doi.org/10.26630/jk.v9i3.1083

Umesh, M., Choudhury, D. D., Shanmugam, S., Ganesan, S., Alsehli, M., Elfasakhany, A., & Pugazhendhi, A. (2021). Eggshells biowaste for hydroxyapatite green synthesis using extract piper betel leaf - Evaluation of antibacterial and antibiofilm activity. Environmental Research, 200. https://doi.org/10.1016/j.envres.2021.111493

Wolkoff, P. (2018). Indoor air humidity, air quality, and health – An overview. International Journal of Hygiene and Environmental Health, 221(3), 376–390. https://doi.org/10.1016/j.ijheh.2018.01.015

Wolkoff, P. (2024). Indoor air humidity revisited: Impact on acute symptoms, work productivity, and risk of influenza and COVID-19 infection. International Journal of Hygiene and Environmental Health, 256, 114313. https://doi.org/10.1016/j.ijheh.2023.114313

World Health Organization. (2020). Pembersihan dan disinfeksi permukaan lingkungan dalam konteks COVID-19: Panduan Interm. World Health Organization. https://cdn.who.int/media/docs/default-source/searo/indonesia/covid19/pembersihan-dan-disinfeksi-permukaan-lingkungan-dalam-konteks-covid-19.pdf?sfvrsn=2842894b_2

World Health Organization. (2024). Household air pollution. World Health Organization. https://doi.org/10.1093/oso/9780197662526.003.0026

Yushananta, P., & Ahyanti, M. (2021). The Effectiveness of Betle Leaf (Piper betle L.) Extract as a Bio- pesticide for Controlled of Houseflies (Musca domestica L.). Open Access Macedonian Journal of Medical Sciences, 9(E), 895–900. https://doi.org/10.3889/oamjms.2021.6886

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Published

2024-12-31

How to Cite

Nugraheni, R. M., Sudaryanto, S., Prasetyawati, N. D., & Mulyaningsih, T. (2024). Effect of Green Betle Leaf Extract (Piper betle L.) on Indoor Microbiological Quality. Ruwa Jurai: Jurnal Kesehatan Lingkungan, 18(3), 121–127. https://doi.org/10.26630/rj.v18i3.4566

Issue

Vol. 18 No. 3 (2024)

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