Visualisasi Matriks Biofilm Eschericia coli dengan Media Bacteriological Peptone, Sucrose dan Ethanol
DOI:
https://doi.org/10.26630/jk.v9i1.730Keywords:
Biofilm, Esherichia coli, EPSAbstract
Escherichia coli is a bacterium which is found in the intestines as a normal flora. In 2011, WHO (World Health Organization) showed that 14% of Children's Deaths that is caused by Escherichia coli. On the other hand, Escherichia colicauses 30-40% incidence of Nosocomial Urinary Tract Infection was associated with the use of urinary catheters. In extreme conditions, Escherichia coli can create biofilms, such as at reduced nutrient conditions, pH 7-7, and temperature 200C - 400C. Biofilm is formed from Escherichia coli that is contained EPS (extracellular polymeric substances). It has function as protector of bacterial cells. EPS contains glucuronic acid and Manosa. The formation of Biofilm can extend treatment process of patients and can cause chronic and recurrent inflammatory process. Aims this study is to know the characteristics of Escherichia coli biofilm and visualize the biofilm produced using sucrose 2%, 5% ethanol and 25% Peptone bacteriological in a culturing process. This study is an experimental and use microorganisms Escherichia coli which is cultured in Laboratory of Microbiology, University of Lampung and used Scanning Electron Microscope (SEM) for visualization and Aurum (Au) for coating. Based on the findings of observation under SEM (Scanning Electron Microscope), Escherichia coli has a thick layer as a biofilm, particularly in Bacteriological Peptone 25%. Therefore, bacterial cell colonies sheltered perfect from various antibacterial. Conclusion: Escherichia coli perfectly can create biofilms. For advanced research, can find the inhibitor of biofilm and destroyer of Escherichia coli cells.
References
Dewanti, R., & Wong, A. C. 1995. Influence of culture conditions on biofilm formation by Escherichia coli O157: H7. International journal of food microbiology, 26(2), 147-164.
Donlan, R. M. 2001. Biofilm formation: a clinically relevant microbiological process. Clinical Infectious Diseases, 33(8), 1387-1392.
Donlan, R. M. 2002. Biofilms: Microbial life on Surfaces. Emerging Infectious Diseases. 8: 881-890.
Madigan MT, Martinko JM, Brock TD. 2006. Brock Biology of Microorganism. 11th ED. New Jersey: Pearsom Prentice Hall. p:617-619.
Marlina Dwi. Can Melittin Treat Biofilm of MRSA (Methicillin Resistant Staphylococcus aureus). Result & Discussion. [Thesis]. Australia: Curtin University. p. 1-16 ; 2015.
Mahon CR, Lehman DC, dan Manuselis, George. 2011. Text Book of Diagnostiv Microbiology. 4th Edition. China: Sauders Elsevier.
Maryana Dwi. 2014. Pengaruh Penambahan Sukrosa Terhadap Jumlah Bakteri dan Kesamaan Whey Fermentasi dengan Menggunakan Kombinasi Lactobacillus plantarum dan Lactobacillus acidophilus. Tinjauan Pustaka. [Skripsi]. Makassar: Universitas Hasanuddin.
P. Naves, Leslie A, Roberto Kolter. 2000. Exopolysaccaride Production Is Required for Development of Escherichia coli K-12. Biofilm Architecture. Vol.182. No.12 .p : 3593-3596.
Purwani Eni, Hapsari Nur, Rauf Rusdin. 2009. Respon Hambatan Bakteri Gram Positif Dan Gram Negatif pada Ikan Nila (Oreochromis niloticus) yang Diawetkan dengan Ekstrak Jahe (Zingiber officinale). Jurnal Kesehatan ISSN 1979-7621, Vol. 2, No. 1.
Rachmadi Dedi. 2009. Peranan Probiotik pada Infeksi Saluran Kemih. http://pustaka.unpad,ac.id>2013/12. (Diakses pada 13 Maret 2015).
Stoodley P, Sauer K, Davies DG, Costerton JW. 2002. Biofilms as complex differentiated communities. Annu Rev Microbiol; 56: 187-209.
Sutarma. 2000. Kultur Media Bakteri. Temu Teknis Fungsional non Peneliti. https://s3.amazonaws.com/academia.edu.documents/44344445/ptek00-8.pdf?AWSAccessKeyId=AKIAIWOWYYGZ2Y53UL3A&Expires=1525238070&Signature=CE8Iuf39gMM0YkgXAq8kCbyVxq8%3D&response-content-disposition=inline%3B%20filename%3DPtek00.pdf (Diakses pada 25 Desember 2015).
Suciati A, Muntalif SB. 2013. Dinamika Pertumbuhan Mikroorganisme Yang Berperan Pada Degredasi Biowaste Dalam Reaktor Anaerob Tercurah. [Skripsi]. Bandung. Program Studi Teknik Lingkungan Fakultas Teknik Sipil dan Lingkungan, Institut Teknologi Bandung. http://ftsl.itb.ac.id (Diakses Pada 3 april 2016).
Sukenda, Hadi, P., & Harris, E. 2006. Pengaruh pemberian sukrosa sebagai sumber karbon dan probiotik terhadap dinamika populasi bakteri dan kualitas air media budidaya udang vaname, Litopenaeus vannamei. Effect of Sucrose as Carbon Source and Probiotic Administrations on Bacterial Population Dinamic and Water Quality in White Shrimp, Litopenaeus vannamei Culture. Jurnal Akuakultur Indonesia. 5(2): 179-190
Tamime, A.Y. 2006. Fermented Milks. Blackwell: UK.
Winarno, F.G. 2008. Kimia Pangan dan Gizi. Edisi terbaru. Jakarta: Gramedia Pustaka Utama.
Downloads
Additional Files
Published
Issue
Section
License
Authors who publish in this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY-SA 4.0) that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors can enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.