Kemampuan Sabun Antiseptik Cair yang Mengandung Triclosan yang Terdaftar di BPOM dalam Menghambat Pertumbuhan Bakteri Escherichia coli

Authors

  • Marhamah Marhamah Jurusan Analis Kesehatan, Politeknik Kesehatan Tanjungkarang
  • Sri Ujiani Jurusan Analis Kesehatan, Politeknik Kesehatan Tanjungkarang
  • Maria Tuntun Jurusan Analis Kesehatan, Politeknik Kesehatan Tanjungkarang

DOI:

https://doi.org/10.26630/jk.v10i1.1228

Keywords:

Antiseptic soap, Escherichia coli, Triclosan

Abstract

In the community, there is still circulating hand washing soap containing triclosan and registered with BPOM/Ministry of Health, but the packaging is not printed how many milliliters of water should be added so that the concentration is not effective in killing bacteria on the hand. The purpose of this study was to determine the ability of liquid antiseptic soap containing triclosan registered at BPOM/Ministry of Health in inhibiting the growth of Escherichia coli bacteria and bacteria on the hands. The research was carried out at the Bacteriology Laboratory of the Department of Health Analyst at Tanjungkarang Health Polytechnic and Lampung Veterinary Center in July-November 2017. This type of research was a completely randomized design. Pre-experiment of one group pre-post-test, the test method of Total Plate Numbers (ALT). Test uses concentrations of 2%, 4%, 6%, 8% and 10% with 5 repetitions. The results showed the strength of liquid antiseptic soap containing triclosan with a concentration of 2%, 4%, 6%, 8% and 10% including the criteria of being moderate and effective in killing bacteria on the hand with a decrease in germ number 96.18%. The Anova test obtained p-value 0,000, meaning that there was an effect of the concentration of liquid antiseptic soap on the growth of Escherichia coli bacteria. The LSD test results showed a significant difference between each liquid antiseptic soap concentration (p-value 0,000-0,024) in inhibiting bacterial growth. The results of the T-test showed that there were differences in the number of germs (ALT) in the hands before and after hand washing with liquid antiseptic soap.

Author Biography

Marhamah Marhamah, Jurusan Analis Kesehatan, Politeknik Kesehatan Tanjungkarang

Google Scholar ID

References

Burton M, Cobb E, Donachie P, Judah G, Curtis V, Schmidt WP. (2011). The Effect of Handwashing with Water or Soap on Bacterial Contamination of Hands. Int. J. Environ. Res. Public Health. Vol 8. 97-104.

Campbell, N. A, J. B. Reece dan L.A, Mitchell. (1996). Biologi. Edisi Kelima, Jilid I, Terjemaham dari Biology. Oleh. Rahayu Lestari, dkk. Jakarta: Erlangga.

Chandra. (2007). Pengantar Kesehatan Lingkungan. Jakarta: Penerbit Buku Kedokteran EGC.

Franklin TJ, Snow GA. (2005). Biochemistry and Molecular Biology of Antimicrobial Drug Action. 6th Edition. New York: Springer Science & Business Media Inc.

Fardiaz. (1993). Analisis Mikrobiologi Pangan. Jakarta: PR. Raja Grafindo Persada.

Girou E, Loyeau S, Legrand P, Oppein F, Brun-Buisson C. (2002). Efficacy of Handrubbing with Alcohol Based Bolution Versus Standard Handwashing With Aanticeptic soap: Randomised Clinical Trial. BMJ. vol 325

Notoatmodjo, Soekidjo. (2005). Metodologi Penelitian Kesehatan. Jakarta: Rineka Cipta.

Nugraha A.P. (2016). Bahaya Dibalik Sabun Antibakteri yang Mengandung Triclosan. http://www.putma.com. Diakses pada 4 Februari 2017.

Pelezar, Michael. & Chan. (2005). Dasar-Dasar Mikrobiologi. Jakarta: Universitas Indonesia.

Soemarno. (2000). Isolasi dan Identifikasi Bakteri Klinik. Yogyakarta: Akademi Analis Kesehatan Yogyakarta DEPKES RI.

SS. (2001). Disinfection, Sterilization, and Preservation 5th Edition. USA: Lippincott Williams & Wilkins.

Tjay, Tan Hoan, Kirana Rahardja. (2007). Obat-Obat Penting Khasiat, Penggunaan dan Efek-Efek Sampingnya. Jakarta: PT. Elex Media Komputindo Kelompok Kompas.

Virgiawan.D.P. (2014), Uji Daya Hambat Sabun Antiseptik Cair Terhadap Pertumbuhan bakteri Echerichia coli, KTI. Poltekkes Tanjungkarang, Bandar Lampung.

Widodo, Wahyu, Sri Sumarsih. (2007). Jarak Kepyar. Yogyakarta: Kanisius.

Downloads

Published

11-05-2019

Issue

Vol. 10 No. 1 (2019): Jurnal Kesehatan

Section

Articles

License

Authors who publish in this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY-SA 4.0) that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
  2. Authors can enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.