Potensi Kombinasi Pemberian Ekstrak Rosella dan Pare Menurunkan Kadar Glukosa Darah dan Meningkatkan Kolesterol-HDL pada Tikus Model Sindrom Metabolik
DOI:
https://doi.org/10.26630/jkmsaw.v15i2.3598Keywords:
Rosella, Pare, Fasting Blood Glucose, HDL.Abstract
Latar Belakang: Sindrom metabolik merupakan salah satu masalah kesehatan yang meningkat saat ini. Sindrome metabolik akan meningkatkan risiko diabetes melitus tipe 2 dan kardiovaskuler. Penyakit ini menjadi ancaman kesehatan sehingga dibutuhkan kombinasi obat dalam menangani berbagai gangguan disfungsi. Terapi obat kimia tidak cukup menyembuhkan atau menghentikan perkembangan penyakit. Tanaman Rosella dan pare memiliki aktifitas sebagai hipoglikemik dan antilipidemik. Tujuan: Untuk mengetahui efek kombinasi ekstrak rosella dan pare dalam menurunkan kadar glukosa darah puasa dan meningkatkan kadar HDL terhadap tikus model sindrom metabolik. Metode: Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan sampel penelitian dibagi dalam 5 kelompok perlakuan. Analisis data dengan uji paired T Test. Tahapan pelaksanaannya yaitu pengkondisian tikus sindrom metabolik. Hasil: Pada kelompok PII didapatkan penurunanan kadar glukosa darah puasa yang signifikan setelah intervensi sebesar 37,43 % (p value = 0,001). Pada kelompok PII didapatkan peningkatan kadar HDL yang signifikan setelah interveni 48,95 % (p value = 0,005) Simpulan: Potensi kombinasi pemberian ekstrak rosella dan pare mampu menurunkan kadar glukosa darah puasa dan meningkatkan kadar HDL tikus model sindrom metabolik. Simpulan: Kadar glukosa darah puasa tikus model sindrom metabolik menurun dan kadar HDL meningkat setelah diberikan intervensi kombinasi ekstrak rosella dan pare. Tahap lanjutan studi adalah untuk mengetahui uji toksisitas akut penggunaan rosella dan pare. Abstract: The potency of Combination of Rosella and Bitter Gourd Extracts to Reduce Blood Glucose Levels and Increase HDL-Cholesterol in Metabolic Syndrome Model Rats. Background: Metabolic syndrome is one of the increasing health problems today. The metabolic syndrome will increase the risk of type 2 diabetes mellitus and cardiovascular disease. This disease is a health threat that requires a combination of drugs to treat various dysfunction disorders. Chemical drug therapy is not enough to cure or stop the progression of the disease. Rosella and bitter melon plants have activities as hypoglycemic and antilipidemic. Purposes: To determine the effect of a combination of rosella and bitter melon extracts in reducing fasting blood glucose levels and increasing HDL levels in metabolic syndrome model rats. Methods: The research design used was a completely randomized design (CRD) with the research sample divided into 5 treatment groups. Data analysis with paired T-test. The stages of implementation are conditioning of metabolic syndrome rats. Result: In group PII, there was a significant decrease in fasting blood glucose levels after intervention by 37.43% (p-value = 0.001). In group PII, there was a significant increase in HDL levels after the intervention of 48.95% (p-value = 0.005) Conclusion: After receiving a mixture of rosella and bitter melon extracts, rats with the metabolic syndrome model had lower fasting blood glucose levels and higher levels of HDL. The next stage of the study was to determine the acute toxicity test of using rosella and bitter melon.References
Adib-Hajbaghery, M., Fattahi Ardakani, M., Sotoudeh, A., & Asadian, A. (2021). Prevalence of complementary and alternative medicine (CAM) among diabetic patients in Eastern Mediterranean country members of the World Health Organization (WHO): A review. Journal of Herbal Medicine, 29(May), 100476. https://doi.org/10.1016/j.hermed.2021.100476
Berruezo, S., Ferreyra, S., Fontana, A., de Paola, M., Pérez, M. B., Scapini, C., Cremer, C., & Castro, C. (2022). Mulinum spinosum root extract, rich in antioxidant compounds, mitigates harmful effects in mice with diet-induced metabolic syndrome. Phytomedicine Plus, 2(1), 100169. https://doi.org/10.1016/j.phyplu.2021.100169
Biradar, R. A., Singh, D. P., & Prasad, J. B. (2021). Burden of increased blood glucose due to modifiable risk faktors among men in India. Diabetes and Metabolic Syndrome: Clinical Research and Reviews, 15(3), 725–732. https://doi.org/10.1016/j.dsx.2021.03.007
Do Vale Moreira, N. C., Hussain, A., Bhowmik, B., Mdala, I., Siddiquee, T.,
Fernandes, V. O., Montenegro Júnior, R. M., & Meyer, H. E. (2020). Prevalence of Metabolic Syndrome by different definitions, and its association with type 2 diabetes, pre-diabetes, and cardiovascular disease risk in Brazil. Diabetes and Metabolic Syndrome: Clinical Research and Reviews, 14(5), 1217–1224. https://doi.org/10.1016/j.dsx.2020.05.043
Febriani, A., Dikman, I. M., & Annisa Ullya Rasyida. (2022). Pengaruh Ekstrak Daun Jeruju (Acanthus ilicifolius) terhadap Penurunan Kadar LDL Darah pada Tikus Putih (Rattus norvegicus) Jantan Galur Wistar Diabetes yang Diinduksi Aloksan. Jambi Medical Journal, 10(4), 485–492. https://doi.org/10.30649/obj.v3i2.50
Gesteiro, E., MegÃa, A., Guadalupe-Grau, A., Fernandez-Veledo, S., Vendrell, J., & González-Gross, M. (2021). Early identification of metabolic syndrome risk: A review of reviews and proposal for defining pre-metabolic syndrome status. Nutrition, Metabolism and Cardiovascular Diseases, 31(9), 2557–2574. https://doi.org/10.1016/j.numecd.2021.05.022
Ghafuri, S., Ghaderi, E., Fahami, Y., Rajabnia, M., & Naleini, S. N. (2019). Epidemiologic study of type 2 diabetes mellitus and metabolic syndrome in rural population of kurdistan province, Iran, in 2011–2017. Diabetes and Metabolic Syndrome: Clinical Research and Reviews, 13(3), 1689–1697. https://doi.org/10.1016/j.dsx.2019.03.037
Orno, T. G., Madiuw, D., & Siauta, V. A. (2022). Edukasi dan Deteksi Dini Sindrom Metabolik pada Masyarakat Desa Waai Kecamatan Salahutu. Karya Kesehatan Siwalima, 1(1), 1-6. https://doi.org/10.54639/kks.v1i1.720
Haufe, S., Kerling, A., Protte, G., Bayerle, P., Stenner, H. T., Rolff, S., Sundermeier, T., Kück, M., Ensslen, R., Nachbar, L., Lauenstein, D., Böthig, D., Bara, C., Hanke, A. A., Terkamp, C., Stiesch, M., Hilfiker-Kleiner, D., Haverich, A., & Tegtbur, U. (2019). Telemonitoring-supported exercise training, metabolic syndrome severity, and work ability in company employees: a randomised controlled trial. The Lancet Public Health, 4(7), e343–e352. https://doi.org/10.1016/S2468-2667(19)30075-1
Hussain, M. S., Jahan, N., Or Rashid, M. M., Hossain, M. S., Chen, U., &
Rahman, N. (2019). Antihyperlipidemic screening and plasma uric acid reducing potential of Momordica charantia seeds on Swiss albino mice model. Heliyon, 5(5), e01739. https://doi.org/10.1016/j.heliyon.2019.e01739
Ijazati, A. (2021). Pengaruh pemberian jus pare (momordica charantia (l) terhadap gula darah dan histopatologi pankreas pada tikus putih jantan (ratttus norvegicus) diabetes (Doctoral dissertation, Universitas Andalas). http://scholar.unand.ac.id/96135/
Innih, S. O., Eze, I. G., & Omage, K. (2022). Evaluation of the haematinic, antioxidant and anti-atherosclerotic potential of Momordica charantia in cholesterol-fed experimental rats. Toxicology Reports, 9(March), 611–618. https://doi.org/10.1016/j.toxrep.2022.03.042
Kesavadev, J., Misra, A., Saboo, B., Aravind, S. R., Hussain, A., Czupryniak, L., & Raz, I. (2021). Diabetes & Metabolic Syndrome : Clinical Research & Reviews Blood glucose levels should be considered as a new vital sign indicative of prognosis during hospitalization. Diabetes & Metabolic Syndrome: Clinical Research & Reviews, 15(1), 221–227. https://doi.org/10.1016/j.dsx.2020.12.032
Li, Y., Zhao, L., Yu, D., Wang, Z., & Ding, G. (2018). Metabolic syndrome prevalence and its risk faktors among adults in China: A nationally representative cross-sectional study. PLoS ONE, 13(6), 1–16. https://doi.org/10.1371/journal.pone.0199293
Lisius Marbun, R. (2019). Potential of Pare Momordica charantia L as a Lowering Level Blood Cholesterol. Jiksh, 10(2), 188–192. https://doi.org/10.35816/jiskh.v10i2.147
Mahwish, Saeed, F., Arshad, M. S., Nisa, M. U., Nadeem, M. T., & Arshad, M. U. (2017). Hypoglycemic and hypolipidemic effects of different parts and formulations of bitter gourd (Momordica Charantia). Lipids in Health and Disease, 16(1), 1–11. https://doi.org/10.1186/s12944-017-0602-7
Mani, P., Ren, H. Y., Neeland, I. J., McGuire, D. K., Ayers, C. R., Khera, A., & Rohatgi, A. (2017). The association between HDL particle concentration and incident metabolic syndrome in the multi-ethnic Dallas Heart Study. Diabetes and Metabolic Syndrome: Clinical Research and Reviews, 11, S175–S179. https://doi.org/10.1016/j.dsx.2016.12.028
Maryusman, T. k, Dien, C. D., & Mail, S. H. (2021). Uji Efektifitas Sinbiotik Kefir Tepung Pisang Batu Lipid Tikus Model Sindrom Metabolik. National Nutrition Journal, 16(3), 296–305. https://www.e-journal.unair.ac.id/MGI/article/view/19940
Mongi, R., Simbala, H. E. I., & De Queljoe, E. (2019). Uji aktivitas penurunan kadar gula darah ekstrak etanol daun pinang yaki (areca vestiaria) terhadap tikus putih jantan galur wistar (rattus norvegicus) yang diinduksi aloksan. Pharmacon, 8(2), 449. https://doi.org/10.35799/pha.8.2019.29312
Murningtyas, F., Larasati, M., Rahmawati, A., & Prihatin, S. (2020). Besar Risiko Faktor Fisiologis dan Faktor Perilaku terhadap Kejadian Sindrom Metabolik The Great Risk of Physiology and Behavioral Faktors with Metabolic Syndrome Incidents. Jurnal Riset Gizi, 8(1), 11. https://ejournal.poltekkes-smg.ac.id/ojs/index.php/jrg/article/view/5152
Novera Herdiani, E. A. W. (2020). Efek Antioksidan Ekstrak Kelopak Rosella Terhadap Glukosa Darah Tikus Diabetes Mellitus Tipe 2. An-Nadaa: Jurnal Kesehatan Masyarakat, 7(2), 89. https://doi.org/10.31602/ann.v7i2.3321
Ohsugi, M., Eiki, J. ichi, Iglay, K., Tetsuka, J., Tokita, S., & Ueki, K. (2021). Comorbidities and complications in Japanese patients with type 2 diabetes mellitus: Retrospective analyses of J-DREAMS, an advanced electronic medical records database. Diabetes Research and Clinical Practice, 178, 108845. https://doi.org/10.1016/j.diabres.2021.108845
Orgah, J. O., He, S., Wang, Y., Jiang, M., Wang, Y., Orgah, E. A., Duan, Y.,
Zhao, B., Zhang, B., Han, J., & Zhu, Y. (2020). Pharmacological potential of the combination of Salvia miltiorrhiza (Danshen) and Carthamus tinctorius (Honghua) for diabetes mellitus and its cardiovascular complications. Pharmacological Research, 153(December 2019), 104654. https://doi.org/10.1016/j.phrs.2020.104654
Pengpid, S., & Peltzer, K. (2020). Prevalence and correlates of the metabolic syndrome in a cross-sectional community-based sample of 18–100 year-olds in Morocco: Results of the first national STEPS survey in 2017. Diabetes and Metabolic Syndrome: Clinical Research and Reviews, 14(5), 1487–1493. https://doi.org/10.1016/j.dsx.2020.07.047
Putra, O. M., Jawi, I. M., & Satriyasa, B. K. (2019). Uji efektivitas ekstrak etanol air kelopak bunga Hibiscus sabdariffa linn terhadap profil lipid tikus hiperlipidemia. Intisari Sains Medis, 10(2), 273–278. https://doi.org/10.15562/ism.v10i2.189
Putriningtyas, N. D., Permatasari, I., Oktaviani, D., Raha, A. S., & Wahyuningsih, S. (2020). Red dragon fruit (Hylocereus spp.) peel marmalade effectively improve blood glucose and lipid profile of hypercholesterolemic wistar rats. Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition), 9(1), 61–67. https://doi.org/10.14710/jgi.9.1.61-67
Salsabila, D. M., Maryusman, T., & Fatmawati, I. (2020). Pengaruh sinbiotik kefir tepung pisang batu terhadap kadar glukosa darah tikus sindrom metabolik. Jurnal Bioteknologi & Biosains Indonesia (JBBI), 7(1), 18–27. https://doi.org/10.29122/jbbi.v7i1.3730
Sari, H., Nurmaulia, N., Wahyudi, W., & Fahdi, F. (2020). Uji efektivitas antikolesterol kombinasi ekstrak etanol daun afrika (gymnanthemum amygdalina del.) Dengan ekstrak etanol daun kelapa sawit (elaeis guineensis jacq.) Pada tikus hiperkolesterol. Jurnal Penelitian Farmasi & Herbal, 2(2), 1–7. https://doi.org/10.36656/jpfh.v2i2.206
Sasongko, H., Lestari, R. G., Indasari, R. D., & Wulandari, R. D. (2022). The Hypolipidemic Effect of Mountain Papaya and Bitter Melon Fruit Ethanolic Extract in Diabetic Rats. 07(03), 1–13. https://doi.org/10.22146/jtbb.75349
Tandi, J., Na’i, A., & Basilingan, A. (2019). Uji Efek Kombinasi Eeds Dan Dpw Terhadap Penurunan Kadar Kolesteroltotal Dan Glukosa Darah Tikusputih Jantan Hiperkolesterolemia-Diabetes. Jurnal Farmasi Medica/Pharmacy Medical Journal (PMJ), 2(1). https://doi.org/10.35799/pmj.2.1.2019.23607
Tatto, D., Dewi, N. P., & Tibe, F. (2017). Efek Antihiperkolesterol dan Antihiperglikemik Daun Ceremai (Phyllantus acidus (L.) Skeels) pada Tikus Putih Jantan (Rattus norvegicus) Hiperkolesterol Diabetes. Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal), 3(2), 157–164. https://doi.org/10.22487/j24428744.0.v0.i0.8769
Wahyuni, D. U., & Sunoko, H. R. (2022). Pengaruh pemberian infusa kelopak bunga rosella (hibiscus sabdariffa l.) Sebagai penurun kadar glukosa darah pada mencit putih jantan (mus musculus galur swiss-webster) yang diinduksi glukosa. Generics: Journal of Research in Pharmacy, 2(1), 54-60. https://doi.org/10.14710/genres.v2i1.11269
Yehualashet, F. A., Tegegne, E. T., Ayele, A. D., & Takele, W. W. (2021). Do adult patients with diabetes mellitus living in rural part of Ethiopia and having poor social support have disproportionately poor self-care practice? A systematic review and meta-analysis. Primary Care Diabetes, 15(4), 642–652. https://doi.org/10.1016/j.pcd.2021.02.010
Yunita Listiani, Veriza Aprilita, E. M. (2019). Uji efek antidiabetes kombinasi ekstrak etanol biji kedelai (glycine max l.) Dan minyak zaitun (olea europea) terhadap mencit putih jantan yang diinduksi aloksan. Jurnal Ilmiah Bakti Farmasi, IV(2), 43–50. https://ejournal.stifibp.ac.id/index.php/jibf/article/view/56
Zhi-hao, Z. H. A. O., Ai-min, S. H. I., Rui, G. U. O., Hong-zhi, L. I. U., Hui, H. U., & Qiang, W. A. N. G. (2022). Protective effect of high-oleic acid peanut oil and extra-virgin olive oil in rats with diet-induced metabolic syndrome by regulating branched-chain amino acids metabolism. Journal of Integrative Agriculture, 21(3), 878–891. https://doi.org/10.1016/S2095-3119(21)63851-0
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