Perbedaan Karakteristik Koloni Bakteri Staphylococcus aureus Pada Media Agar Darah Domba dan Media Agar Darah Manusia
DOI:
https://doi.org/10.26630/jak.v11i1.3202Keywords:
Media for sheep's blood, Media for human blood, Staphylococcus aureusAbstract
Sheep blood agar is the standard medium for culturing pathogenic bacteria, as an alternative, human blood agar media is used as an alternative from transfusion. This study aims to observe the differences in the characteristics of Staphylococcus aureusbacteria on sheep blood agar media and human blood agar media which was carried out at BBLK Palembang. This type of research was conducted experimentally with a total sample of 32 and analyzed statistically. Bacterial suspension derived from the pure strain of Staphylococcus aureus ATCC 25923. The colony characteristics were observed macroscopically including colony morphology, colony number and colony
diameter. The average value of the number of colonies of Staphylococcus aureus bacteria on sheep blood agar and human blood agar media were 173 and 148, while the colony diameters were 2.5 mm and 2 mm, respectively. The results of the research data on the number of colonies were tested with an unpaired T test with p value = 0.001 and colony diameter data was tested with the Mann Whitney alternative test with p = 0.031, which means that there is a statistically significant difference between the characteristics of the Staphylococcus aureusbacteria colony on the media. sheep blood agar and human blood agar media.
References
Candrasari, A., Romas, M. A., &Astuti, O. R. (2011). Uji Daya Antimikroba Ekstrak Etanol Daun Sirih Merah (Piper Crocatum Ruiz &Pav.) Terhadap Pertumbuhan Staphylococcus aureus ATCC 6538, Eschericia coliAtcc 11229 Dan Candida albicansAtcc 10231 Secara In Vitro. Biomedika, 5(1), 9–16. https://doi.org/10.23917/biomedika.v4i1.258
Hiaranya.(2017). Mikrobiologi.Bahan Ajar Keperawatan Gigi.Jakarta Selatan.
Karimela, E. J., Ijong, F. G., &Dien, H. A. (2017).Karakteristik Staphylococcus Aureus Yang Di Isolasi Dari Ikan Asap Pinekuhe Hasil Olahan Tradisional Kabupaten Sangihe Characteristics of Staphylococcus aureus Isolated Smoked Fish Pinekuhe from Traditionally Processed from Sangihe District.Jphpi, 20(1).https://doi.org/10.17844/jphpi.2017.20.1.356
Krihariyani, D., Woelansari, E. D., &Kurniawan, E. (2016).Pola Pertumbuhan Staphylococcus aureus Pada Media Agar Darah Manusia Golongan O, AB, dan Darah Domba Sebagai Kontrol.Jurnal Ilmu Dan Teknologi Kesehatan, 3(2), 1–10. https://www.poltekkesjakarta3.ac.id/ejurnalnew/index.php/jitek/article/view/96
Niederstebruch. (2017). A Suitable Blood Agar Containing Human Blood Especially For The Use Inlaboratories Of Developing Countries.Original Article. Vol 11 (5):399-406.
Novard, M. F. A., Suharti, N., & Rasyid, R. (2019).Gambaran Bakteri Penyebab Infeksi Pada Anak Berdasarkan Jenis Spesimen dan Pola Resistensinya di Laboratorium RSUP Dr. M. Djamil Padang Tahun 2014-2016.Jurnal Kesehatan Andalas, 8(2S), 26.https://doi.org/10.25077/jka.v8i2s.955
Novita, R. I. D., &Febrianti, I. (2019).Pemanfaatan Penggunaan Darah Donor Yang Telah Kadaluwarsa Untuk Pembuatan Agar Darah Pada Pertumbuhan Staphylococcus aureus.Jurnal Pengelolaan Laboratorium Pendidikan, 1(2), 64–69. https://doi.org/10.14710/jplp.1.2.64-69
Nurhidayanti, N. (2019). Pemanfaatan Darah Sisa Transfusi Dalam Pembuatan Media Bap Untuk Pertumbuhan Bakteri Streptococcus pyogenes.Indobiosains, 1(2), 63.https://doi.org/10.31851/indobiosains.v1i2.3189
Purbowarsito, H. (2011). Uji Bakteriologis Air Sumur di Kecamatan Semampir Surabaya.75, 39–40.
Septiani, S., Dewi, E. N., &Wijayanti, I. (2017).Aktivitas Antibakteri Ekstrak Lamun (CymodoceaRotundata) Terhadap Bakteri Staphylococcus aureus dan Escherichia coli (Antibacterial Activities of Seagrass Extracts (Cymodocearotundata) Against Staphylococcus aureus and Escherichia coli).Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology, 13(1), 1. https://doi.org/10.14710/ijfst.13.1.1-6
Yusmaniar. (2017). Mikrobiologi Dan Parasitologi.Bahan Ajar Farmasi
Downloads
Published
Issue
Section
License
Jurnal Analisis Kesehatan is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Based on a work at https://ejurnal.poltekkes-tjk.ac.id/index.php/JANALISKES.
